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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been observed in many tumors, which ends up in chromosome instability, and therefore may well add to tumorigenesis. This protein was discovered to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a important job in advertising cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are actually explained. Two supplemental splice variants were being claimed but comprehensive nucleotide sequence details isn't however accessible.
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Blocking peptides are peptides that bind especially to your goal antibody and block antibody binding. These peptide usually incorporates the epitope acknowledged from the antibody.
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This guideline outlines even more feasible optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This guideline outlines further more probable optimization of mobile and immunoassay parameters to be sure the absolute best outcomes are...
Antibodies bound to the blocking peptide no longer bind into the epitope to the focus on protein. By comparing the staining within the blocked antibody as opposed to the antibody on your own, you can see which staining is precise.
Goal Information Cyclin E belongs into the hugely conserved cyclin family members, whose members are characterised by a dramatic periodicity in protein abundance from the cell cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins show distinct expression and degradation designs which contribute on the temporal coordination of each and every mitotic function. Cyclin E sorts a fancy with and capabilities as being a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S transition. This protein accumulates within the G1-S stage boundary which is degraded as cells development by means of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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